| 质粒名称: | 7TFP CDH1 reporter |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91704 |
| 存储实验室: | Bob Weinberg |
|
载体骨架基本信息 |
|
| 载体名称: | 7TFP |
| 载体出品公司: | Roel Nusse (Addgene plasmid # 24308) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9061 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，荧光素酶 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CDH1 promoter |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 243 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CDH1 |
| 标签/融合蛋白: | luciferase（主干上的C末端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf1（销毁后未知） |
| 3' 克隆位点: | Bmt1（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | LucNRev |
|
文献引用方法 |
|
| 材料和方法部分: | 7TFP CDH1 reporter was a gift from Bob Weinberg (Addgene plasmid # 91704 ; http://n2t.net/addgene:91704 ; RRID:Addgene_91704) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 7TFP CDH1 reporter 9174 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION lentiviral luciferase reporter containing CDH1 promoter.
ACCESSION .
VERSION .
KEYWORDS 7TFP CDH1 reporter
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9174)
AUTHORS Pattabiraman DR, Bierie B, Kober KI, Thiru P, Krall JA, Zill C,
Reinhardt F, Tam WL, Weinberg RA
TITLE Activation of PKA leads to mesenchymal-to-epithelial transition and
loss of tumor-initiating ability.
JOURNAL Science. 2016 Mar 4;351(6277):aad3680. doi: 10.1126/science.aad3680.
PUBMED 26941323
REFERENCE 2 (bases 1 to 9174)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9174
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 325..356
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 412..421
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 418..2070
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(484..502)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
primer_bind complement(2089..2109)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2094..2423
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2274..2409
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2433..3032
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2433..2452)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2929..2949
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 3099..3687
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3152..3172)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3570..3581)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3774..4007
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4079..4200
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4116..4135)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4170..4189
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 4240..4375
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(4395..4414)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4396..4414)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4424..4441)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4424..4440)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4433..4455)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4582..5037
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4669..4688)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4879..4900
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 5063..5167
/gene="bla"
/label=AmpR promoter
CDS 5168..6028
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5386..5405)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6199..6787
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6688..6707
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6941..6958
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7075..7096
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7111..7141
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7149..7165
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7154..7176
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7173..7189
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7173..7189
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 7208..7228
/label=T3
/note="T3 promoter, forward primer"
promoter 7210..7228
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 7256..7482
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 7483..7663
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7710..7835
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8328..8561
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 9057..9174
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 atcgatcacg agactagcct cctagcccgg gctcgagcag ctgaagcttg catgcctgca
61 ggcgtccgcg ctgctgattg gctgtggccg gcaggtgaac cctcagccaa tcagcggtac
121 ggggggcggt gcctccgggg ctcacctggc tgcagccacg caccccctct cagtggcgtc
181 ggaactgcaa agcacctgtg agcttgcgga agtcagttca gactccagcc cgctccagcc
241 cggcccgacc cgaccgcacc cggcgcctgc cctcgctcgg cgtccccggc agccaccgct
301 agcccgggct cgagatctag actctagagg gtatataatg gaagctcgaa ttccagcttg
361 gcattccggt actgttggta aaaagcttgg cattccggta ctgttggtaa agccaccatg
421 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc
481 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
541 tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt
601 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
661 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
721 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg
781 cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
841 aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt
901 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
961 gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct
1021 actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat
1081 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
1141 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
1201 gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag
1261 attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt
1321 gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag
1381 gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg
1441 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
1501 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
1561 ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat
1621 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
1681 gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg
1741 attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac
1801 cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc
1861 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
1921 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
1981 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
2041 gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc gaccctgtgg aatgtgtgtc
2101 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
2161 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
2221 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
2281 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
2341 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
2401 ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc acggtgcgcc
2461 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
2521 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
2581 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
2641 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
2701 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
2761 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
2821 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
2881 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
2941 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
3001 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
3061 ccgaaaggag cgcacgaccc catgcatcgc cagtcgacaa tcaacctctg gattacaaaa
3121 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
3181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
3241 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
3301 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
3361 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
3421 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
3481 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
3541 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
3601 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
3661 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
3721 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
3781 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
3841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
3901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
3961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
4021 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
4081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
4141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
4201 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
4261 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
4321 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
4381 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
4441 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
4501 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
4561 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
4621 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
4681 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
4741 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
4801 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
4861 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
4921 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
4981 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
5041 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
5101 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
5161 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5221 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5281 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5341 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5401 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5461 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5521 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5581 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5641 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5701 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5761 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
5821 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
5881 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5941 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
6001 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
6061 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
6121 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
6181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6301 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6601 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6781 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6841 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
6901 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
6961 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
7021 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
7081 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
7141 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
7201 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
7261 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
7321 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
7381 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
7441 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
7501 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
7561 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
7621 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
7681 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
7741 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
7801 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
7861 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
7921 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
7981 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
8041 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
8101 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
8161 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
8221 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
8281 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
8341 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
8401 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
8461 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
8521 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
8581 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
8641 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
8701 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
8761 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
8821 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
8881 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
8941 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
9001 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
9061 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
9121 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa tttt
//
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